Open AccessPeriodic acid-Schiff staining parallels the immunoreactivity seen by direct immunofluorescence in autoimmune skin diseases
Author(s) -
Ana Maria Abreu Velez,
Yulieth Alexandra Upegui-Zapata,
Michael S. Howard
Publication year - 2016
Publication title -
north american journal of medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2250-1541
pISSN - 1947-2714
DOI - 10.4103/1947-2714.179132
Subject(s) - medicine , staining , pathology , direct fluorescent antibody , autoantibody , erythema multiforme , bullous pemphigoid , periodic acid–schiff stain , pemphigoid , dermatology , immunofluorescence , h&e stain , pemphigus , antibody , immunology
In many countries and laboratories, techniques such as direct immunofluorescence (DIF) are not available for the diagnosis of skin diseases. Thus, these laboratories are limited in the full diagnoses of autoimmune skin diseases, vasculitis, and rheumatologic diseases. In our experience with these diseases and the patient's skin biopsies, we have noted a positive correlation between periodic acid-Schiff (PAS) staining and immunofluorescence patterns; however, these were just empiric observations. In the current study, we aim to confirm these observations, given the concept that the majority of autoantibodies are glycoproteins and should thus be recognized by PAS staining.