Open AccessIn vivo technique for cellular calcium waves documentation: A light microscopy method
Author(s) -
Abrahám A. Embí,
Manuel Menes
Publication year - 2013
Publication title -
north american journal of medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2250-1541
pISSN - 1947-2714
DOI - 10.4103/1947-2714.115769
Subject(s) - calcium , in vivo , microscopy , von kossa stain , calcium in biology , confocal microscopy , confocal laser scanning microscopy , biophysics , pathology , biomedical engineering , chemistry , medicine , microbiology and biotechnology , biology , biochemistry , enzyme , alkaline phosphatase
We are introducing a novel in vivo technique to document cellular calcium deposits, which reflect a snapshot of the effect of calcium wave propagation. This technique however is not advocated enough to replace the accuracy and resolution of the confocal laser technique. Light microscopy equipment, calcium chelators and a histological calcium staining kit are essential.