Open AccessStatistical optimization of culture conditions for expression of recombinant humanized anti-EpCAM single-chain antibody using response surface methodology
Author(s) -
Aidin Behravan,
Atieh Hashemi
Publication year - 2021
Publication title -
research in pharmaceutical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.685
H-Index - 29
eISSN - 1735-9414
pISSN - 1735-5362
DOI - 10.4103/1735-5362.310522
Subject(s) - epithelial cell adhesion molecule , recombinant dna , titer , antibody , microbiology and biotechnology , lac operon , cell culture , cell , chemistry , cancer research , gene , biology , immunology , biochemistry , genetics
The epithelial cell adhesion molecule (EpCAM), is one of the first cancer- associated markers discovered. Its overexpression in cancer stem cells, epithelial tumors, and circulating tumor cells makes this molecule interesting for targeted cancer therapy. So, in recent years scFv fragments have been developed for EpCAM targeting.