Open AccessNerve growth factor-basic fibroblast growth factor poly-lactide co-glycolid sustained-release microspheres and the small gap sleeve bridging technique to repair peripheral nerve injury
Author(s) -
Ming Li,
Ting-Min Xu,
Dianying Zhang,
Xiaomeng Zhang,
Feng Rao,
Sizheng Zhan,
Man Ma,
Chen Xiong,
Xiaofeng Chen,
Yanhua Wang
Publication year - 2023
Publication title -
neural regeneration research/neural regeneration research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.93
H-Index - 38
eISSN - 1876-7958
pISSN - 1673-5374
DOI - 10.4103/1673-5374.344842
Subject(s) - sciatic nerve , nerve growth factor , basic fibroblast growth factor , growth factor , peripheral nerve injury , fibroblast , nerve injury , nerve guidance conduit , biomedical engineering , regeneration (biology) , fibroblast growth factor , sciatic nerve injury , chemistry , medicine , materials science , microbiology and biotechnology , anatomy , surgery , biology , in vitro , biochemistry , receptor
We previously prepared nerve growth factor poly-lactide co-glycolid sustained-release microspheres to treat rat sciatic nerve injury using the small gap sleeve technique. Multiple growth factors play a synergistic role in promoting the repair of peripheral nerve injury; as a result, in this study, we added basic fibroblast growth factors to the microspheres to further promote nerve regeneration. First, in an in vitro biomimetic microenvironment, we developed and used a drug screening biomimetic microfluidic chip to screen the optimal combination of nerve growth factor/basic fibroblast growth factor to promote the regeneration of Schwann cells. We found that 22.56 ng/mL nerve growth factor combined with 4.29 ng/mL basic fibroblast growth factor exhibited optimal effects on the proliferation of primary rat Schwann cells. The successfully prepared nerve growth factor-basic fibroblast growth factor-poly-lactide-co-glycolid sustained-release microspheres were used to treat rat sciatic nerve transection injury using the small gap sleeve bridge technique. Compared with epithelium sutures and small gap sleeve bridging alone, the small gap sleeve bridging technique combined with drug-free sustained-release microspheres has a stronger effect on rat sciatic nerve transfection injury repair at the structural and functional level.