Open AccessDried Rehmannia root protects against glutamate-induced cytotoxity to PC12 cells through energy metabolism-related pathways
Author(s) -
Yong Liu,
Lei Liu,
Xixiang Ying,
Wenjuan Wei,
Chao Han,
Yang Liu,
Chunhui Han,
Aijing Leng,
Jingyun Ma,
Jing Liu
Publication year - 2017
Publication title -
neural regeneration research/neural regeneration research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.93
H-Index - 38
eISSN - 1876-7958
pISSN - 1673-5374
DOI - 10.4103/1673-5374.213556
Subject(s) - glutamate receptor , neuroprotection , apoptosis , chemistry , pharmacology , lactate dehydrogenase , cytotoxic t cell , biochemistry , nad+ kinase , microbiology and biotechnology , biology , enzyme , in vitro , receptor
Rehmannia has been shown to be clinically effective in treating neurodegenerative diseases; however, the neuroprotective mechanisms remain unclear. In this study, we established a model of neurodegenerative disease using PC12 cytotoxic injury induced by glutamate. The cells were treated with 20 mM glutamate in the absence or presence of water extracts of dried Rehmannia root of varying concentrations (70%, 50% and 30%). The different concentrations of Rehmannia water extract significantly increased the activity of glutamate-injured cells, reduced the release of lactate dehydrogenase, inhibited apoptosis, increased the concentrations of NADH, NAD and ATP in cells, ameliorated mitochondrial membrane potential, and reduced the levels of light chain 3. Taken together, our findings demonstrate that Rehmannia water extracts exert a cytoprotective effect against glutamate-induced PC12 cell injury via energy metabolism-related pathways.