Open AccessFermented Chinese formula Shuan-Tong-Ling attenuates ischemic stroke by inhibiting inflammation and apoptosis
Author(s) -
Zhigang Mei,
Lingjing Tan,
Jinfeng Wang,
Xiaoli Li,
Weifeng Huang,
Hongbo Zhou
Publication year - 2017
Publication title -
neural regeneration research/neural regeneration research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.93
H-Index - 38
eISSN - 1876-7958
pISSN - 1673-5374
DOI - 10.4103/1673-5374.202946
Subject(s) - medicine , panax notoginseng , traditional medicine , angelica sinensis , salvia miltiorrhiza , pharmacology , radix (gastropod) , apoptosis , inflammation , allopurinol , traditional chinese medicine , immunology , botany , biochemistry , chemistry , biology , pathology , alternative medicine
The fermented Chinese formula Shuan-Tong-Ling is composed of radix puerariae ( Gegen ), salvia miltiorrhiza ( Danshen ), radix curcuma ( Jianghuang ), hawthorn ( Shanzha ), salvia chinensis ( Shijianchuan ), sinapis alba ( Baijiezi ), astragalus ( Huangqi ), panax japonicas ( Zhujieshen ), atractylodes macrocephala koidz ( Baizhu ), radix paeoniae alba ( Baishao ), bupleurum ( Chaihu ), chrysanthemum ( Juhua ), rhizoma cyperi ( Xiangfu ) and gastrodin ( Tianma ), whose aqueous extract was fermented with lactobacillus , bacillus aceticus and saccharomycetes . Shuan-Tong-Ling is a formula used to treat brain diseases including ischemic stroke, migraine, and vascular dementia. Shuan-Tong-Ling attenuated H 2 O 2 -induced oxidative stress in rat microvascular endothelial cells. However, the potential mechanism involved in these effects is poorly understood. Rats were intragastrically treated with 5.7 or 17.2 mL/kg Shuan-Tong-Ling for 7 days before middle cerebral artery occlusion was induced. The results indicated Shuan-Tong-Ling had a cerebral protective effect by reducing infarct volume and increasing neurological scores. Shuan-Tong-Ling also decreased tumor necrosis factor-α and interleukin-1β levels in the hippocampus on the ischemic side. In addition, Shuan-Tong-Ling upregulated the expression of SIRT1 and Bcl-2 and downregulated the expression of acetylated-protein 53 and Bax. Injection of 5 mg/kg silent information regulator 1 (SIRT1) inhibitor EX527 into the subarachnoid space once every 2 days, four times, reversed the above changes. These results demonstrate that Shuan-Tong-Ling might benefit cerebral ischemia/reperfusion injury by reducing inflammation and apoptosis through activation of the SIRT1 signaling pathway.