Open AccessRhesus monkey neural stem cell transplantation promotes neural regeneration in rats with hippocampal lesions
Author(s) -
Lijuan Yang,
Hui Bian,
Fan Yang,
Zhengbo Wang,
Hualin Yu,
Yuanye Ma,
Feng Chen
Publication year - 2016
Publication title -
neural regeneration research/neural regeneration research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.93
H-Index - 38
eISSN - 1876-7958
pISSN - 1673-5374
DOI - 10.4103/1673-5374.191221
Subject(s) - hippocampal formation , neural stem cell , transplantation , neuroscience , stem cell , pathology , biology , neurosphere , hippocampus , lesion , corpus callosum , anatomy , adult stem cell , medicine , embryonic stem cell , microbiology and biotechnology , gene , biochemistry
Rhesus monkey neural stem cells are capable of differentiating into neurons and glial cells. Therefore, neural stem cell transplantation can be used to promote functional recovery of the nervous system. Rhesus monkey neural stem cells (1 × 10 5 cells/μL) were injected into bilateral hippocampi of rats with hippocampal lesions. Confocal laser scanning microscopy demonstrated that green fluorescent protein-labeled transplanted cells survived and grew well. Transplanted cells were detected at the lesion site, but also in the nerve fiber-rich region of the cerebral cortex and corpus callosum. Some transplanted cells differentiated into neurons and glial cells clustering along the ventricular wall, and integrated into the recipient brain. Behavioral tests revealed that spatial learning and memory ability improved, indicating that rhesus monkey neural stem cells noticeably improve spatial learning and memory abilities in rats with hippocampal lesions.