Open AccessActivation of the Notch signaling pathway promotes neurovascular repair after traumatic brain injury
Author(s) -
Qi-shan Ran,
Yun-hu Yu,
Xiao-Hong Fu,
Yuanchao Wen
Publication year - 2015
Publication title -
neural regeneration research/neural regeneration research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.93
H-Index - 38
eISSN - 1876-7958
pISSN - 1673-5374
DOI - 10.4103/1673-5374.162758
Subject(s) - notch signaling pathway , hes3 signaling axis , microbiology and biotechnology , progenitor cell , angiogenesis , signal transduction , gene knockdown , endothelial stem cell , neurovascular bundle , biology , neuroscience , cancer research , medicine , stem cell , anatomy , apoptosis , biochemistry , in vitro
The Notch signaling pathway plays a key role in angiogenesis and endothelial cell formation, but it remains unclear whether it is involved in vascular repair by endothelial progenitor cells after traumatic brain injury. Therefore, in the present study, we controlled the Notch signaling pathway using overexpression and knockdown constructs. Activation of the Notch signaling pathway by Notch1 or Jagged1 overexpression enhanced the migration, invasiveness and angiogenic ability of endothelial progenitor cells. Suppression of the Notch signaling pathway with Notch1 or Jagged1 siRNAs reduced the migratory capacity, invasiveness and angiogenic ability of endothelial progenitor cells. Activation of the Notch signaling pathway in vivo in a rat model of mild traumatic brain injury promoted neurovascular repair. These findings suggest that the activation of the Notch signaling pathway promotes blood vessel formation and tissue repair after brain trauma.