Open AccessChronic neuroprotective effects of low concentration lithium on SH-SY5Y cells: possible involvement of stress proteins and gene expression
Author(s) -
Riadh Nciri,
Ezzeddine Bourogâa,
Samira Jbahi,
Mohamed Salah Allagui,
Abdelfattah Elfeki,
Christian Vincent,
F. Croute
Publication year - 2014
Publication title -
neural regeneration research/neural regeneration research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.93
H-Index - 38
eISSN - 1876-7958
pISSN - 1673-5374
DOI - 10.4103/1673-5374.131578
Subject(s) - sh sy5y , neuroprotection , microbiology and biotechnology , heat shock protein , hsp27 , hsp70 , protein kinase a , gene expression , protein phosphatase 2 , protein kinase c , phosphatase , lithium (medication) , western blot , kinase , chemistry , biology , phosphorylation , biochemistry , gene , cell culture , endocrinology , pharmacology , genetics , neuroblastoma
To investigate the molecular mechanism underlying the neuroprotective effect of lithium on cells, in this study, we exposed SH-SY5Y cells to 0.5 mmol/L lithium carbonate (Li2CO2) for 25-50 weeks and then detected the expression levels of some neurobiology related genes and post-translational modifications of stress proteins in SH-SY5Y cells. cDNA arrays showed that pyruvate kinase 2 (PKM2) and calmodulin 3 (CaM 3) expression levels were significantly down-regulated, phosphatase protein PP2A expression was lightly down-regulated, and casein kinase II (CK2), threonine/tyrosine phosphatase 7 (PYST2), and dopamine beta-hydroxylase (DBH) expression levels were significantly up-regulated. Besides, western blot analysis of stress proteins (HSP27, HSP70, GRP78 and GRP94) showed an over-expression of two proteins: a 105 kDa protein which is a hyper-phosphorylated isoform of GRP94, and a 108 kDa protein which is a phosphorylated tetramer of HSP27. These results suggest that the neuroprotective effects of lithium are likely related to gene expressions and post-translational modifications of proteins cited above.