Open AccessHepatitis c virus genotype 4 replication in the hepatocellular carcinoma cell line HepG2/C3A
Author(s) -
Medhat K. Shier,
Mohammad S. ElWetidy,
Hebatallah H. Ali,
Mohammad M. AlQattan
Publication year - 2016
Publication title -
the saudi journal of gastroenterology/saudi journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.608
H-Index - 32
eISSN - 1998-4049
pISSN - 1319-3767
DOI - 10.4103/1319-3767.182461
Subject(s) - virology , hepatitis c virus , flow cytometry , viral replication , hepatocellular carcinoma , cell culture , biology , virus , immunofluorescence , microbiology and biotechnology , antibody , immunology , cancer research , genetics
The lack of a reliable cell culture system allowing persistent in vitro hepatitis C virus (HCV) propagation is still restraining the search for novel antiviral strategies. HepG2 cells transfection with HCV allows for viral replication. However, the replication is weak presumably because of HepG2 lack of miRNA-122, which is essential for viral replication. Other agents such as polyethylene glycol (PEG) and dimethyl sulfoxide (DMSO) have been shown to increase the efficiency of infection with other viruses. This study included comparison of HCV genotype 4 5'UTR and core RNA levels and HCV core protein expression at different time intervals in the absence or presence of PEG and/or DMSO postinfection.