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The antinociceptive activity of ethanolic extract of the plant bark of Dalbergia sissoo (Roxb.) was investigated using tail flick method on Wistar rats. Three different dose levels (300, 500, and 1000 mg/kg) in 0.5% carboxyl methyl cellulose (CMC) were administered by p.o. route. The antinociceptive activities of the all doses were compared with that of the standard drug asprin (300 mg/kg) administered by p.o. route and the results were found to be significant (P &lt; 0.01). At the above doses, the extract exhibited significant and dose-dependent antinociceptive activity. Phytochemical investigation of the ethanolic extract indicated the presence of carbohydrates, proteins, amino acids, phenolic compounds, and flavanoids. The antinociceptive activity of the bark extract of D. sissoo may be due to the presence of phytochemical constituents such as flavanoids. The acute toxicity study revealed that ethanolic extract was not toxic up to 3000 mg/kg body weight.

Phytochemical investigation and evaluation of antinociceptive activity of ethanolic extract of Dalbergia sissoo (Roxb.) bark