Open AccessEvaluation of cytotoxicity of aqueous extract of Graviola leaves on squamous cell carcinoma cell-25 cell lines by 3-(4,5-dimethylthiazol-2-Yl) -2,5-diphenyltetrazolium bromide assay and determination of percentage of cell inhibition at G2M phase of cell cycle by flow cytometry: An in vitro study
Author(s) -
Visveswaraiah Paranjyothi Magadi,
Venkatadasappa Ravi,
Arpitha Anantharaju,
Litha,
Kikkerilakshminarayana Kumaraswamy,
K. Manjunath
Publication year - 2015
Publication title -
contemporary clinical dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.289
H-Index - 21
eISSN - 0976-237X
pISSN - 0976-2361
DOI - 10.4103/0976-237x.169863
Subject(s) - annona muricata , flow cytometry , cytotoxicity , cell cycle , cell culture , mtt assay , cell growth , cell , biology , microbiology and biotechnology , in vitro , biochemistry , botany , genetics
Malignancies constitute a wide variety of disorders having high mortality and morbidity rates. Current protocols for management include surgical intervention, chemotherapy, and radiation which possess numerous adverse effects. Many phytochemicals are available with anticancer properties similar to anticancer drugs. Major benefit of these compounds is apparent lack of toxicity to normal tissues. Graviola (botanical name: Annona Muricata) contain bioactive compound "annonaceous acetogenins" known for anticancer activity on cancer cell lines.