Open AccessDevelopment of siRNA lipoplexes for intracellular delivery in lung cancer cells
Author(s) -
Nirav Khatri,
Ambikanandan Misra
Publication year - 2012
Publication title -
journal of pharmacy and bioallied sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 36
eISSN - 0976-4879
pISSN - 0975-7406
DOI - 10.4103/0975-7406.94115
Subject(s) - intracellular , cytotoxicity , lung cancer , cancer research , small interfering rna , gene delivery , liposome , in vitro , cell culture , cell , cancer , chemistry , genetic enhancement , medicine , transfection , microbiology and biotechnology , biology , gene , pathology , biochemistry , genetics
Lung cancer occurrence throughout the world and its unsatisfactory treatment has drawn significant attention from many medical agencies. A genomic approach utilizing siRNA against target gene may resolve this problem. Present investigation includes siRNA lipoplex formulation and its in-vitro characterisation in lung cancer cell line for intracellular uptake and cytotoxicity. Accumulation of siRNA inside the cell as well as less cytotoxicity of prepared carrier system signifies potential of prepared siRNA lipoplex in the treatment of lung cancer.