Open AccessIsolation and purification of fungal pathogen (Macrophomina phaseolina) induced chitinase from moth beans (Phaseolus aconitifolius)
Author(s) -
Neelima Garg,
Himanshu Gupta
Publication year - 2010
Publication title -
journal of pharmacy and bioallied sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 36
eISSN - 0976-4879
pISSN - 0975-7406
DOI - 10.4103/0975-7406.62708
Subject(s) - chitinase , macrophomina phaseolina , sephadex , sodium dodecyl sulfate , enzyme , phaseolus , molecular mass , enzyme assay , biochemistry , chitin , biology , ammonium sulfate precipitation , size exclusion chromatography , chromatography , in vivo , chemistry , microbiology and biotechnology , botany , chitosan
Chitinase (EC 3.2.1.14) is one of the major pathogenesis-related proteins, which is a polypeptide that accumulates extracellularly in infected plant tissue. An attempt was made to isolate and purify the chitanase enzyme using moth beans as an enzyme source.