Open AccessBergenin determination in different extracts by high-performance thin-layer chromatographic densitometry
Author(s) -
M. S. Y. Khan,
Washim Khan,
Wasim Ahmad,
Mhaveer Singh,
Sayeed Ahmad
Publication year - 2015
Publication title -
journal of pharmacy and bioallied sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 36
eISSN - 0976-4879
pISSN - 0975-7406
DOI - 10.4103/0975-7406.168024
Subject(s) - bergenin , chromatography , densitometry , rhizome , aglycone , silica gel , chemistry , high performance thin layer chromatography , saxifragaceae , traditional medicine , thin layer chromatography , botany , glycoside , biology , stereochemistry , medicine
Bergenin is an active secondary metabolite, found in Bergenia ligulata, family Saxifragaceae, which is an important medicinal plant used in the traditional system of medicine. It is distributed throughout the South and East Asia and some European countries, usually growing on high altitude in the Himalayan region and known as Pashanbhed (meaning "to break the stone"). The rhizome of B. ligulata has been used since long time in different traditional formulations of kidney and liver disorders. Due to its exhaustive use in the traditional system, it is commonly adulterated with the rhizome of other plants which do not contain its chemical marker bergenin. Hence, we developed high-performance thin-layer chromatographic (HPTLC) method for quantification of bergenin in B. ligulata which can be used for its quality control.