Open AccessA novel validated stability indicating high performance liquid chromatographic method for estimation of degradation behavior of ciprofloxacin and tinidazole in solid oral dosage
Author(s) -
Bhupendrasinh Vaghela,
Surendra Singh Rao
Publication year - 2013
Publication title -
journal of pharmacy and bioallied sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 36
eISSN - 0976-4879
pISSN - 0975-7406
DOI - 10.4103/0975-7406.120082
Subject(s) - tinidazole , chromatography , chemistry , forced degradation , high performance liquid chromatography , dosage form , phosphoric acid , solvent , acetonitrile , degradation (telecommunications) , ciprofloxacin hydrochloride , ciprofloxacin , reversed phase chromatography , organic chemistry , biochemistry , antibiotics , telecommunications , metronidazole , computer science
The objective of current investigation was to study the degradation behavior of Ciprofloxacin and Tinidazole. The study was performed as per International Conference on Harmonization recommended stress condition. A novel stability-indicating reverse phase HPLC method was developed for the determination of Ciprofloxacin and Tinidazole purity in the presence of its impurities and forced degradation products. This method is also capable to separate placebo peaks as well in pharmaceutical dosage forms. The solid oral dosage form was subjected to the stress conditions such as oxidative, acid, base hydrolysis, heat and photolytic degradation.