Open AccessAnalysis of common bean (Phaseolus vulgaris L., genotype BAT93) calmodulin cDNA using computational tools
Author(s) -
Kassim Amelia,
Jasvin Singh,
Farida Habib Shah,
Subhash Janardhan Bhore
Publication year - 2015
Publication title -
pharmacognosy research
Language(s) - English
Resource type - Journals
eISSN - 0976-4836
pISSN - 0974-8490
DOI - 10.4103/0974-8490.150536
Subject(s) - phaseolus , genotype , calmodulin , complementary dna , biology , computational biology , botany , genetics , biochemistry , gene , enzyme
Common bean (Phaseolus vulgaris L.) is an important part of the human diet and serves as a source of natural products. Identification and understanding of genes in P. vulgaris is important for its improvement. Characterization of expressed sequence tags (ESTs) is one of the approaches in understanding the expressed genes. For the understanding of genes expression in P. vulgaris pod-tissue, research work of ESTs generation was initiated by constructing cDNA libraries using 5-day and 20-day old bean-pod-tissues. Altogether, 5972 cDNA clones were isolated to have ESTs. While processing ESTs, we found a transcript for calmodulin (CaM) gene. It is an important gene that encodes for a calcium-binding protein and known to express in all eukaryotic cells. Hence, this study was undertaken to analyse and annotate it.