Open AccessComparison of DNA extraction protocols for Mycobacterium Tuberculosis in diagnosis of tuberculous meningitis by real-time polymerase chain reaction
Author(s) -
Rajeev Thakur,
Satyam Sarma,
Renu Goyal
Publication year - 2011
Publication title -
journal of global infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 25
eISSN - 0974-8245
pISSN - 0974-777X
DOI - 10.4103/0974-777x.91057
Subject(s) - polymerase chain reaction , dna extraction , taqman , nucleic acid , dna , real time polymerase chain reaction , mycobacterium tuberculosis , detection limit , extraction (chemistry) , tuberculous meningitis , chromatography , biology , tuberculosis , virology , microbiology and biotechnology , medicine , chemistry , pathology , gene , biochemistry
Several nucleic acid amplification techniques are available for detection of Mycobacterium tuberculosis (MTB) in pulmonary and extrapulmonary samples, but insufficient data are available on the diagnostic utility of these techniques in tubercular meningitis where bacilli load is less. The success of final amplification and detection of nucleic acid depends on successful extraction of DNA from the organism.