Open AccessIsolation of epithelial cells from acrylic removable dentures and gender identification by amplification of SRY gene using real time PCR
Author(s) -
Renjith George,
Gopu Sriram,
TR Saraswathi,
B Sivapathasundharam
Publication year - 2010
Publication title -
journal of forensic odontology
Language(s) - English
Resource type - Journals
ISSN - 0974-2948
DOI - 10.4103/0974-2948.71055
Subject(s) - testis determining factor , dentures , polymerase chain reaction , saliva , amelogenin , real time polymerase chain reaction , microbiology and biotechnology , dna , genomic dna , biology , typing , isolation (microbiology) , gene , y chromosome , genetics , dentistry , medicine , bioinformatics , biochemistry
This study evaluates the usefulness of acrylic dentures as the source of DNA for forensic analysis. Thirty-eight samples (21 males and 17 females) were collected and stored for different time periods. The epithelial cells adhered to the dentures were retrieved and the genomic DNA was extracted. All the samples yielded sufficient amount of DNA for analysis irrespective of the storage time. Gender determination was done by amplification of the sex determining region on the Y chromosome (SRY) using real-time polymerase chain reaction with 100% accuracy, within minimal time. With this study, we conclude that saliva-stained acrylic dentures can act as a source of forensic DNA and co-amplification of SRY gene with other routine sex typing markers will give unambiguous gender identification.