Open AccessSensitivity of individual and mini-pool nucleic acid testing assessed by dilution of hepatitis B nucleic acid testing yield samples
Author(s) -
Kabita Chatterjee,
Nitin Agarwal,
Poonam Coshic,
Mayuri Borgohain,
Sourit Chakroborty
Publication year - 2014
Publication title -
asian journal of transfusion science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.262
H-Index - 22
eISSN - 1998-3565
pISSN - 0973-6247
DOI - 10.4103/0973-6247.126684
Subject(s) - nat , serial dilution , nucleic acid , medicine , nucleic acid test , hepatitis b virus , dilution , viral load , chromatography , serology , virology , virus , biology , immunology , chemistry , antibody , covid-19 , biochemistry , pathology , mathematics , statistics , alternative medicine , disease , physics , infectious disease (medical specialty) , thermodynamics
For nucleic acid testing (NAT) of blood donations, either the blood samples can be pooled together in a batch of six or eight prior to testing (mini-pool-NAT [MP-NAT]), or the tests can be run on every individual sample (individual donor-NAT [ID-NAT]). It has been debated in various studies whether pooling of samples results in decreased sensitivity of detection as the volume of individual samples gets lesser in a pool. The objective of this study was to investigate the effect of dilution on the sensitivity of tests.