Open AccessRhesus-D zygosity and hemolytic disease of fetus and newborn
Author(s) -
Mostafa Moghaddam,
Amirali Naghi,
Fatemeh Hassani,
Sedighe Amini
Publication year - 2013
Publication title -
asian journal of transfusion science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.262
H-Index - 22
eISSN - 1998-3565
pISSN - 0973-6247
DOI - 10.4103/0973-6247.115584
Subject(s) - medicine , zygosity , fetus , disease , antibody , pregnancy , obstetrics , antigen , immunology , polymerase chain reaction , hemolytic disease of the newborn (abo) , gene , genetics , biology
Alloimmunization against the Rhesus-D (RhD) antigen still remains as a major cause of hemolytic disease of fetus and newborn (HDFN). Determination of paternal RhDzygosity is performed by molecular testing and is valuable for the management of alloimmunized pregnant women. A 30-year-old pregnant woman with AB negative blood group presented with two consecutive abortions and no history of blood transfusion. By application of the antibody screening, identification panel, and selected cells, she was found to be highly alloimmunized. RhDzygosity was performed on her partner and was shown to be homozygous for RhD. The sequence- specific priming-polymerase chain reaction used in this report is essential to establish whether the mother requires an appropriate immunoprophylaxis or the fetus is at risk of HDFN.