Open AccessA rapid and sensitive method for EphB4 identification as a diagnostic and therapeutic biomarker in invasive breast cancer
Author(s) -
Mona Moshayedi,
Farnaz Barneh,
Shaghayegh Haghjooy Javanmard,
Hamid Mirmohammad Sadeghi,
Nahid Eskandari,
Ali Mohammad Sabzghabaee
Publication year - 2016
Publication title -
journal of cancer research and therapeutics/journal of cancer research and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 39
eISSN - 0973-1482
pISSN - 1998-4138
DOI - 10.4103/0973-1482.147254
Subject(s) - breast cancer , phosphorylation , cancer research , cancer , biomarker , receptor , cell culture , stimulation , ca 15 3 , microbiology and biotechnology , medicine , biology , chemistry , ca15 3 , biochemistry , genetics
In the roadmap to design diagnostic and therapeutic markers for breast cancer, EphB4 is of special interest due to its multiple roles in tumor initiation, progression and invasion. The aim of present study was to characterize a rapid and sensitive ELISA-based method to measure EphB4 level and its phosphorylation status following stimulation with its ligand, ephrinB2, in an invasive breast cancer cell line.