The investigation of epsilon toxin effects on different cancerous cell lines and its synergism effect with methotrexate | Zendy

Sadegh Hasannia | Zendy; AzinGholami Shekarsaraei | Zendy; Nazanin Pirooznia | Zendy; Fariba Ataiee | Zendy

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The investigation of epsilon toxin effects on different cancerous cell lines and its synergism effect with methotrexate

Author(s) -

Sadegh Hasannia,

AzinGholami Shekarsaraei,

Nazanin Pirooznia,

Fariba Ataiee

Publication year - 2014

Publication title -

journal of cancer research and therapeutics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 39

eISSN - 0973-1482

pISSN - 1998-4138

DOI - 10.4103/0973-1482.131338

Subject(s) - cytotoxicity , ic50 , toxin , chemistry , pharmacology , drug , chemotherapy , methotrexate , drug delivery , in vitro , toxicity , mtt assay , biochemistry , medicine , immunology , organic chemistry

The overall goal of this study is to use a bacterial toxin as drug delivery agents for chemotherapy drugs and overcome the development of resistance to these medicines. COR-L105 and MDA-MB 231 which are epithelial-like were used in this study. Cytotoxicity assays were performed by 3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) as metabolic indicator. The toxin was essential to kill 50% (CT50) and IC 50 value (inhibition growth value) for methotrexate were determined as optical density at 540 nm. Epsilon toxin-loaded PLGA nanoparticles were prepared using non-aqueous technique. Surface morphology, in vitro drug release, and encapsulation efficiency of the nanoparticles was determined.

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