Open AccessThe investigation of epsilon toxin effects on different cancerous cell lines and its synergism effect with methotrexate
Author(s) -
Azin Gholami Shekarsaraei,
Sadegh Hasannia,
Nazanin Pirooznia,
Fariba Ataiee
Publication year - 2014
Publication title -
journal of cancer research and therapeutics/journal of cancer research and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 39
eISSN - 0973-1482
pISSN - 1998-4138
DOI - 10.4103/0973-1482.131338
Subject(s) - cytotoxicity , ic50 , toxin , chemistry , pharmacology , drug , chemotherapy , methotrexate , drug delivery , in vitro , toxicity , mtt assay , biochemistry , medicine , immunology , organic chemistry
The overall goal of this study is to use a bacterial toxin as drug delivery agents for chemotherapy drugs and overcome the development of resistance to these medicines. COR-L105 and MDA-MB 231 which are epithelial-like were used in this study. Cytotoxicity assays were performed by 3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) as metabolic indicator. The toxin was essential to kill 50% (CT50) and IC 50 value (inhibition growth value) for methotrexate were determined as optical density at 540 nm. Epsilon toxin-loaded PLGA nanoparticles were prepared using non-aqueous technique. Surface morphology, in vitro drug release, and encapsulation efficiency of the nanoparticles was determined.