Open AccessSimultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD
Author(s) -
Jin Bae Won,
Jin Yeul,
Young Ran Um,
Choong Je
Publication year - 2010
Publication title -
pharmacognosy magazine
Language(s) - English
Resource type - Journals
eISSN - 0976-4062
pISSN - 0973-1296
DOI - 10.4103/0973-1296.62896
Subject(s) - paeoniflorin , chromatography , glycyrrhizin , chemistry , calibration curve , detection limit , gradient elution , high performance liquid chromatography , cinnamic acid , medicine , organic chemistry
A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C(18) column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R(2) > 0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 mug/ml and 0.13 to 1.11 mug/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%.