Open AccessEconomic methods used in fabrication of tissue microarray: A pilot study
Author(s) -
Sahana Srinath,
Rohit Kumar Kendole,
Pavana Gopinath,
Srinath Krishnappa,
S K Vishwanath
Publication year - 2016
Publication title -
journal of oral and maxillofacial pathology/journal of oral and maxillofacial pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.455
H-Index - 25
eISSN - 1998-393X
pISSN - 0973-029X
DOI - 10.4103/0973-029x.180948
Subject(s) - mold , silicone , silicone rubber , materials science , tissue microarray , adhesive , biomedical engineering , fabrication , composite material , immunohistochemistry , pathology , medicine , layer (electronics) , alternative medicine
Tissue microarray (TMA) is a method of harvesting small disks of tissue from a range of standard paraffin tissue blocks and placing them in an array on a recipient paraffin block such that hundreds of cases can be analyzed simultaneously by using only a few microliters of antibody in immunohistochemistry as a single experiment. The TMA construction done with the help of automated tissue arrayer or commercially available rubber molds are expensive. This study involved the fabrication of TMA using rubber-based additional silicone mold constructed in the department and comparison of this method with two other methods of fabricating TMA.