Open AccessMitochondrial-COII sequence polymorphism reflects spatial genetic clustering of Anopheles culicifacies sibling species E in Sri Lanka
Author(s) -
I.N. Harischandra,
R.S. Dassanayake,
B. G. D. N. K. De Silva
Publication year - 2020
Publication title -
journal of vector borne diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.581
H-Index - 41
ISSN - 0972-9062
DOI - 10.4103/0972-9062.310864
Subject(s) - biology , anopheles culicifacies , sri lanka , sibling , genetics , genotyping , microsatellite , malaria , zoology , evolutionary biology , genotype , anopheles , geography , gene , tanzania , psychology , developmental psychology , allele , environmental planning , immunology
Malaria infects around 216 million people annually with estimated 445,000 deaths globally. Anopheles culicifacies is the vector of malaria in Sri Lanka, a complex of five morphologically identical sibling species of which precise identification using DNA-based methods is still under experimentation. This study was carried out in Sri Lanka to observe the utility of BCE-PCR assay based on mitochondrial Cytochrome Oxidase II (COII) developed in India, in sibling species B and E identification in Sri Lanka, to characterize nucleotide and corresponding amino acid sequences of COII region in major vector sibling species E in Sri Lanka and to analyze the spatial distribution pattern of sibling species E in Sri Lanka using microsatellite markers.