Mitochondrial-COII sequence polymorphism reflects spatial genetic clustering of Anopheles culicifacies sibling species E in Sri Lanka | Zendy

B.G.D.N.K. de Silva | Zendy; IreshaN Harischandra | Zendy; RanilS Dassanayake | Zendy

Open Access

Mitochondrial-COII sequence polymorphism reflects spatial genetic clustering of Anopheles culicifacies sibling species E in Sri Lanka

Author(s) -

B.G.D.N.K. de Silva,

IreshaN Harischandra,

RanilS Dassanayake

Publication year - 2020

Publication title -

journal of vector borne diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.581

H-Index - 41

ISSN - 0972-9062

DOI - 10.4103/0972-9062.310864

Subject(s) - biology , anopheles culicifacies , sri lanka , sibling , genetics , genotyping , microsatellite , malaria , zoology , evolutionary biology , genotype , anopheles , geography , gene , tanzania , psychology , developmental psychology , allele , environmental planning , immunology

Malaria infects around 216 million people annually with estimated 445,000 deaths globally. Anopheles culicifacies is the vector of malaria in Sri Lanka, a complex of five morphologically identical sibling species of which precise identification using DNA-based methods is still under experimentation. This study was carried out in Sri Lanka to observe the utility of BCE-PCR assay based on mitochondrial Cytochrome Oxidase II (COII) developed in India, in sibling species B and E identification in Sri Lanka, to characterize nucleotide and corresponding amino acid sequences of COII region in major vector sibling species E in Sri Lanka and to analyze the spatial distribution pattern of sibling species E in Sri Lanka using microsatellite markers.

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