Open AccessPeriodontal pocket as a potential reservoir of high risk human papilloma virus: A pilot study
Author(s) -
MM Dayakar,
А. Н. Шипилова,
Dinesh Gupta
Publication year - 2015
Publication title -
journal of indian society of periodontology (print)/journal of indian society of periodontology (online)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.309
H-Index - 28
eISSN - 0975-1580
pISSN - 0972-124X
DOI - 10.4103/0972-124x.170815
Subject(s) - periodontium , medicine , chronic periodontitis , gingival and periodontal pocket , periodontitis , head and neck squamous cell carcinoma , in situ hybridization , dentistry , messenger rna , head and neck cancer , cancer , biology , gene , genetics
Human papilloma viruses (HPVs) are small DNA viruses that have been identified in periodontal pocket as well as gingival sulcus. High risk HPVs are also associated with a subset of head and neck carcinomas. HPV detection in periodontium has previously involved DNA detection. This study attempts to: (a) Detect the presence or absence of high risk HPV in marginal periodontiun by identifying E6/E7 messenger RNA (mRNA) in cells from samples obtained by periodontal pocket scraping. (b) Detect the percentage of HPV E6/E7 mRNA in cells of pocket scrapings, which is responsible for producing oncoproteins E6 and E7.