Open AccessA comparative evaluation of whole blood total antioxidant capacity using a novel nitroblue tetrazolium reduction test in patients with periodontitis and healthy subjects: A randomized, controlled trial
Author(s) -
Kranti Konuganti,
Hema Seshan,
Sameer Zope,
Wilma Delphine Silvia
Publication year - 2012
Publication title -
journal of indian society of periodontology (print)/journal of indian society of periodontology (online)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.309
H-Index - 28
eISSN - 0975-1580
pISSN - 0972-124X
DOI - 10.4103/0972-124x.106937
Subject(s) - medicine , periodontitis , antioxidant , oxidative stress , antioxidant capacity , chronic periodontitis , whole blood , gastroenterology , immunology , biochemistry , biology
The investigation of disease-related oxidant-antioxidant imbalance is difficult due to the limited availability of specific biomarkers of oxidative stress, and the fact that measurement of individual antioxidant may give misleading picture because antioxidants work in concert through chain breaking reactions. Therefore, analysis of total antioxidant capacity may be the most relevant investigation. As the total blood is continuously exposed to oxidative stress, the aim of the current study was to investigate total blood antioxidant capacity in healthy and periodontitis patients by using novel Nitroblue Tetrazolium reduction test. The study was conducted on 30 non-smoking volunteers with age ranging between 18-40 years. They were categorized into two groups; chronic periodontitis group and healthy group, respectively. Total antioxidant capacity in whole blood was assessed using Nitroblue Tetrazolium reduction test. Results of the present study has shown that the total antioxidant capacity in whole blood in patients with periodontitis was significantly (P<0.005) lower than in control subjects. The reduced total blood antioxidant status in periodontitis subjects warrants further investigation as it may provide a mechanistic link between periodontal disease and several other free radical-associated chronic inflammatory diseases.