Open AccessStudies on the changes in lipid peroxidation and antioxidants in fishes exposed to Hydrogen Sulfide
Author(s) -
R Sreejai,
Jaya Divakaran Sarasamma
Publication year - 2010
Publication title -
toxicology international/indian journal of toxicology
Language(s) - English
Resource type - Journals
eISSN - 0976-5131
pISSN - 0971-6580
DOI - 10.4103/0971-6580.72674
Subject(s) - lipid peroxidation , glutathione , chemistry , malondialdehyde , hydrogen sulfide , oxidative stress , biochemistry , antioxidant , glutathione peroxidase , kidney , endocrinology , medicine , enzyme , biology , organic chemistry , sulfur
In the present aquarium study, Oreochromis mossambicus Peters were exposed to two different concentrations of hydrogen sulfide (H(2)S) (4.9 and 6.6 mg/l), and the changes in lipid peroxidation (LP) products and antioxidants in test fishes were determined in time intervals of 12, 24, 48, 72, and 96 hours. The results showed that with respect to the H(2)S concentration and duration of exposure, alterations were observed in the concentration of LP products and antioxidants in the various organs of the test fishes. Malondialdehyde (MDA) content increased in the liver, gill, kidney, and brain on exposure to H(2)S up to 48 hours, and then the MDA content showed steady value up to 98 hours experimental period. Brain and kidney of fishes showed the maximum increase in concentration of reduced glutathione (GSH) on H(2)S treatment. The gradual decrease in concentration of GSH in the tissues of H(2) S-exposed fishes after 48 to 96 hours compared with the control shows the loss of adaptive mechanisms and the oxidation of GSH to glutathione disulphide (GSSG). Slight increase in the activity of GSH-S-transferase and decrease in activity of GSH peroxidase demonstrated the incapability of the vital organs in neutralizing the peroxides generated in the oxidative stress condition.