Open AccessAn alternative strategy to generate coding sequence of macrophage migration inhibitory factor-2 of Wuchereria bancrofti
Author(s) -
Nikhil Chauhan,
S.L. Hoti
Publication year - 2016
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/0971-5916.180217
Subject(s) - wuchereria bancrofti , biology , genomic dna , coding region , gene , complementary dna , polymerase chain reaction , exon , genetics , macrophage migration inhibitory factor , brugia malayi , microbiology and biotechnology , computational biology , filariasis , immunology , helminths , cytokine
Different developmental stages of Wuchereria bancrofti, the major causal organism of lymphatic filariasis (LF), are difficult to obtain. Beside this limitation, to obtain complete coding sequence (CDS) of a gene one has to isolate mRNA and perform subsequent cDNA synthesis which is laborious and not successful at times. In this study, an alternative strategy employing polymerase chain reaction (PCR) was optimized and validated, to generate CDS of Macrophage migration Inhibitory Factor-2 (wbMIF-2), a gene expressed in the transition stage between L3 to L4.