Open AccessOsteoblast response (initial adhesion and alkaline phosphatase activity) following exposure to a barrier membrane/enamel matrix derivative combination
Author(s) -
S Thangakumaran,
Sabitha Sudarsan,
KV Arun,
Avaneendra Talwar,
Johnson Raja James
Publication year - 2009
Publication title -
indian journal of dental research/indian journal of dental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.277
H-Index - 43
eISSN - 1998-3603
pISSN - 0970-9290
DOI - 10.4103/0970-9290.49048
Subject(s) - enamel matrix derivative , alkaline phosphatase , adhesion , membrane , enamel paint , osteoblast , matrix (chemical analysis) , chemistry , derivative (finance) , biophysics , materials science , dentistry , microbiology and biotechnology , composite material , biochemistry , enzyme , medicine , biology , in vitro , regeneration (biology) , economics , financial economics
The enamel matrix derivative (EMD) has been used in combination with barrier membranes to optimize regeneration in vertical osseous defects. However, the osteoblast response when exposed to the EMD/barrier membrane combination has not yet been evaluated. The osteoblast behavior when exposed to a combination of regenerative materials must be evaluated to fully understand their effect on bone regeneration. Therefore, the present study was undertaken to estimate the initial adhesion and alkaline phosphatase (ALP) activity of an osteoblast cell line (SaOS-2) when exposed to four commercially available resorbable membranes and determine if the addition of EMD had any modulatory effect on osteoblast behavior.