Open AccessComparative study of Candida by conventional and CHROMagar method in non-denture and denture wearers by oral rinse technique
Author(s) -
Shruti Nayak,
B. Kavitha,
Gopu Sriram,
TR Saraswathi,
B Sivapathasundharam,
A L Dorothy
Publication year - 2012
Publication title -
indian journal of dental research/indian journal of dental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.277
H-Index - 43
eISSN - 1998-3603
pISSN - 0970-9290
DOI - 10.4103/0970-9290.104956
Subject(s) - dentures , dentistry , candida albicans , germ tube , medicine , agar , candida tropicalis , agar plate , isolation (microbiology) , corpus albicans , microbiology and biotechnology , biology , bacteria , genetics
Candidal species colonizes the oral cavities of healthy individuals without dentures and also of denture wearers. Soft liners and tissue conditioning materials have been found to support the growth of Candida albicans which may predispose to lesions. The most important and common candidal species are C. albicans, C. tropicalis, and C. glabrata. C albicans is usually isolated from both the fitting surface of the denture and the denture-bearing mucosa of the affected patients. The aim of this study was to isolate, quantify, and speciate candidal species in non-denture wearers (controls) and denture wearers (study group) by the oral rinse technique. Isolation was done using Sabouraud dextrose agar (SDA). Speciation was done using conventional methods like the germ tube test, carbohydrate fermentation test, urease test, as well as the CHROMagar method.