Open AccessOxidative and antioxidative defense system in testicular torsion/detorsion
Author(s) -
Farag A. Elshaari,
R I Elfagih,
Dhastagir Sultan Sheriff,
I. F. Barassi
Publication year - 2011
Publication title -
indian journal of urology/indian journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.333
H-Index - 30
eISSN - 1998-3824
pISSN - 0970-1591
DOI - 10.4103/0970-1591.91436
Subject(s) - testicular torsion , medicine , spermatic cord torsion , oxidative stress , glutathione , reperfusion injury , ischemia , lactate dehydrogenase , pentose phosphate pathway , andrology , endocrinology , biochemistry , surgery , chemistry , enzyme , glycolysis , metabolism
The present study was aimed to assess the early effects of ischemia/reperfusion injury on the oxidants and anti-oxidant defense status in rat testicular tissue by measuring MDA, glucose-6-phosphte dehydrogenase activity and reduced glutathione levels in a designated time frame sequel to reperfusion. Animals were divided randomly into six groups (12 animals per group) in the following order: Group I: Sham-operated control group (Cso) without the application of the torsion. Group 2: Torsion-induced ischemia group (T30 m): Ischemia was induced through the torsion of spermatic cord for a period of 30 min. Group 3: One hour reperfusion group after detorsion (T30 mR1 h). Group 4: Twenty-four hour reperfusion group after detorsion (T30 mR24 h). Group 5: Forty-eight hours reperfusion group after detorsion (T30mR48h). Group 6: One week reperfusion group after detorsion (T30mR1wk).