Open AccessChildhood acute erythroleukemia diagnosis by flow cytometry
Author(s) -
Anjali Sharma,
Gurdeep Buxi,
Ritika Walia,
Rakesh Yadav,
Sunita Sharma
Publication year - 2011
Publication title -
indian journal of pathology and microbiology/indian journal of pathology and microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 31
eISSN - 0974-5130
pISSN - 0377-4929
DOI - 10.4103/0377-4929.77395
Subject(s) - cd117 , immunophenotyping , population , flow cytometry , myelodysplastic syndromes , medicine , polycythemia rubra vera , pathology , bone marrow , acute leukemia , myeloid , acute megakaryoblastic leukemia , leukemia , cd33 , immunology , myeloperoxidase , thrombocytosis , polycythemia vera , precursor cell , myeloid leukemia , cd34 , biology , platelet , cell , stem cell , genetics , environmental health , inflammation
Acute erythroid leukemia in children is very rare. Here is a case of erythroleukemia in a child of age 1.5 years, which was diagnosed on peripheral smear, bone marrow examination, cytochemistry but was confirmed on immunophenotyping. CD45 versus side scatter demonstrated blast population (29%) expressing CD45 of variable intensity (dim to negative). The myeloid nature of blast population showed bright expression of cytoplasmic myeloperoxidase (MPO), heterogenous positivity of CD117 and dim expression of CD13, CD33. These blasts also showed bright positivity for CD71 which showed erythroid nature of blasts. Flow cytometry can be comprehensive enough to completely subtype cases of leukemias/myelodysplastic syndromes, polycythemia rubra vera, non-neoplastic conditions like reactive erythroid hyperplasia following immunosuppressive therapy or viral infections or nutritional deficiencies, unlyzed RBCs or thrombocytosis which may mimic acute erythroid leukemia on flow cytometry.