Open AccessPreservation of continuous cell lines at -85°C: A low-cost alternative for resource limited countries
Author(s) -
Baijayantimala Mishra,
S R Ragini,
I L Kashiv,
Radha Kanta Ratho
Publication year - 2010
Publication title -
indian journal of pathology and microbiology/indian journal of pathology and microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 31
eISSN - 0974-5130
pISSN - 0377-4929
DOI - 10.4103/0377-4929.72068
Subject(s) - vero cell , cell culture , monolayer , limiting , virus , chemistry , biology , virology , biochemistry , engineering , mechanical engineering , genetics
Cell culture is the most popular method of virus propagation because of its high sensitivity. However, the need of high cost liquid nitrogen for storage of cell lines is one of the main limiting factor for its widespread use in developing countries.