Open AccessTransport and storage of sputum specimen by using cetylpyridinium chloride for isolation of mycobacteria
Author(s) -
Pal N,
Babita Sharma,
Bharti Malhotra,
Suman Rishi
Publication year - 2009
Publication title -
indian journal of pathology and microbiology/indian journal of pathology and microbiology
Language(s) - English
Resource type - Journals
eISSN - 0974-5130
pISSN - 0377-4929
DOI - 10.4103/0377-4929.44966
Subject(s) - cetylpyridinium chloride , sputum , isolation (microbiology) , significant difference , microbiology and biotechnology , chloride , chemistry , medicine , tuberculosis , gastroenterology , food science , chromatography , pathology , biology , biochemistry , pulmonary surfactant , organic chemistry
Of the 191 sputum specimens that were collected from pulmonary tuberculosis patients, 78.65% (140/178) specimens were culture positive when processed within 48 h by the NaOH method. The culture positivity in the same specimen that were stored with cetylpyridinium chloride (CPC) and processed after 7-8 days was 70.22% (125/178), whereas those stored without CPC and processed by the NaOH method was 46.62% (83/178). The difference in number of positive cultures obtained before storage and after storage (without CPC) was statistically significant (P = 0.001). Culture positivity by the CPC method was comparable with that of NaOH method before storage and the difference was not statistically significant (P = 0.35).