Open AccessImmunofluorescence profile of discoid lupus erythematosus
Author(s) -
Shreekant Bharti,
Sunil Dogra,
Biman Saikia,
Ranjana Minz Walker,
Seema Chhabra,
Uma Nahar Saikia
Publication year - 2015
Publication title -
indian journal of pathology and microbiology/indian journal of pathology and microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 31
eISSN - 0974-5130
pISSN - 0377-4929
DOI - 10.4103/0377-4929.168850
Subject(s) - histopathology , dermoepidermal junction , discoid lupus erythematosus , direct fluorescent antibody , immunofluorescence , medicine , dermatology , lupus erythematosus , pathology , antibody , immunology , dermis
The direct immunofluorescence (DIF) of skin in conjunction with histopathology gives the best diagnostic yield. It is invaluable in confirming the diagnosis of small vessel vasculitides and bullous lesions of the skin and can be used as an additional tool to pinpoint the diagnosis of systemic and localized autoimmune diseases involving the skin. This study was undertaken to analyze the strength of DIF vis-à -vis histopathology in the diagnosis of discoid lupus erythematosus (DLE) and at the same time to elaborate the specific immunofluorescence findings in the lesions of DLE. The clinical profile and cutaneous lesions of 75 patients with DLE are described. DIF was positive in 68% and histopathology in 60% of cases. The most common immunoreactant was IgG at the dermoepidermal junction, followed by IgM and IgA. A conclusive diagnosis of DLE could be achieved satisfactorily in 64 cases (85%) by a combination of the two techniques.