Open AccessRole of multiplex polymerase chain reaction using IS6110 and Protein b for the diagnosis of extra-pulmonary tuberculosis: North India
Author(s) -
Kusum Sharma,
Suma B Appannanavar,
Manish Modi,
Maninder Pal Singh,
Aman Sharma,
Subhash Varma
Publication year - 2015
Publication title -
indian journal of pathology and microbiology/indian journal of pathology and microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 31
eISSN - 0974-5130
pISSN - 0377-4929
DOI - 10.4103/0377-4929.151164
Subject(s) - mycobacterium tuberculosis , multiplex polymerase chain reaction , medicine , polymerase chain reaction , tuberculosis , pulmonary tuberculosis , antigen , biopsy , multiplex , pathology , microbiology and biotechnology , immunology , biology , gene , bioinformatics , biochemistry
Prompt and accurate diagnosis of extra-pulmonary tuberculosis (TB) is highly challenging. Current conventional techniques lack sensitivity and are time-consuming. Here, we report our experience with multiplex polymerase chain reaction (MPCR) using two targets namely IS6110 and protein antigen b in the diagnosis of extra-pulmonary TB.