Open AccessDevelopment and evaluation of flavi-immunoglobulinM capture enzyme-linked immunosorbent assay
Author(s) -
Palani Gunasekaran,
Padma Priya Padmanabhan,
Karthik Ramesh,
Khaleefathullah Sheriff Asadullah,
Mohana Sambasivam,
Kavita Arunagiri,
Kaveri Krishnasamy
Publication year - 2013
Publication title -
indian journal of pathology and microbiology/indian journal of pathology and microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 31
eISSN - 0974-5130
pISSN - 0377-4929
DOI - 10.4103/0377-4929.120391
Subject(s) - flavivirus , serology , dengue virus , virology , dengue fever , serotype , japanese encephalitis , biology , immunoglobulin m , west nile virus , antibody , virus , encephalitis , immunoglobulin g , immunology
In this study, we report the evaluation of In-house flavi virus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA), which can be used as a screening test to determine the infecting flavivirus serotype over the current serological methods. A panel of 88 sera (inclusive of well characterized dengue, Japanese Encephalitis (JE) and West Nile virus (WNV) positive and negative samples tested and confirmed by commercial kit) was used for evaluation of the kit. The sensitivity and specificity of the In-house capture assay versus the commercial kit for the sero-diagnosis of dengue was 100% and 87% respectively, for JE IgM, it was found to be 90% and 100% respectively, and for West Nile it was 87.5% and 90.9%. Based on the study, we concluded that this flavivirus-serotyping ELISA provides rapid results and may be used as an accurate alternate to other serological tests for the specific diagnosis of flavivirus infections.