Open AccessFluorescence in situ hybridization patterns of BCR/ABL1 fusion in chronic myelogenous leukemia at diagnosis
Author(s) -
Poonam Jain,
Mayur Parihar,
Rayaz Ahmed,
Aby Abraham,
Auro Vishwabandya,
Biju George,
Vikram Mathews,
Alok Srivastava,
Vivi M. Srivastava
Publication year - 2012
Publication title -
indian journal of pathology and microbiology/indian journal of pathology and microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 31
eISSN - 0974-5130
pISSN - 0377-4929
DOI - 10.4103/0377-4929.101742
Subject(s) - fluorescence in situ hybridization , breakpoint cluster region , chronic myelogenous leukemia , abl , fusion gene , philadelphia chromosome , derivative chromosome , biology , leukemia , chromosome 22 , pathology , chromosome , chromosomal translocation , microbiology and biotechnology , medicine , genetics , gene , signal transduction , tyrosine kinase
Chronic myelogenous leukemia (CML) is characterised by the t(9;22)(q34;q11.2) which results in the formation of the BCR/ABL1 fusion gene. Occasionally, the t(9;22) may be associated with submicroscopic deletions of chromosomes 9 and/or 22 which appear to be associated with a worse prognosis. Three or four-way variant t(9;22) may also occur. All these changes as well as gain of the Philadelphia chromosome which represents disease progression can be detected by fluorescence in situ hybridization (FISH) analysis. FISH analysis at presentation is used to determine the number of cells with BCR/ABL1 fusion and establish whether the patterns are typical or atypical. Response to therapy can then be monitored by serial testing.