Open AccessBlocking Posttranslational Core Fucosylation Ameliorates Rat Peritoneal Mesothelial Cell Epithelial-Mesenchymal Transition
Author(s) -
Long-Kai Li,
Nan Wang,
Weidong Wang,
Xiangning Du,
Wen Xu,
Wang Ly,
Yiyao Deng,
Dapeng Wang,
Hongli Lin
Publication year - 2017
Publication title -
chinese medical journal/chinese medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 63
eISSN - 2542-5641
pISSN - 0366-6999
DOI - 10.4103/0366-6999.213963
Subject(s) - small interfering rna , fucosylation , signal transduction , epithelial–mesenchymal transition , receptor , microbiology and biotechnology , mapk/erk pathway , gene knockdown , chemistry , platelet derived growth factor receptor , transfection , biology , downregulation and upregulation , cell culture , growth factor , biochemistry , gene , glycoprotein , glycan , genetics
Core fucosylation (CF), catalyzed by α-1,6 fucosyltransferase (Fut8) in mammals, plays an important role in pathological processes through posttranslational modification of key signaling receptor proteins, including transforming growth factor (TGF)-β receptors and platelet-derived growth factor (PDGF) receptors. However, its effect on peritoneal fibrosis is unknown. Here, we investigated its influence on epithelial-mesenchymal transition (EMT) of rat peritoneal mesothelial cells (PMCs) in vitro induced by a high-glucose (HG) culture solution.