
Application of semi-nested polymerase chain reaction targeting internal transcribed spacer region for rapid detection of panfungal genome directly from ocular specimens
Author(s) -
R Bagyalakshmi,
K Lily Therese,
Madhavan Hn
Publication year - 2007
Publication title -
indian journal of ophthalmology/indian journal of ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.542
H-Index - 51
eISSN - 1998-3689
pISSN - 0301-4738
DOI - 10.4103/0301-4738.33037
Subject(s) - polymerase chain reaction , internal transcribed spacer , nested polymerase chain reaction , aspergillus , biology , microbiology and biotechnology , medicine , genetics , ribosomal rna , gene
The incidence of fungal endophthalmitis has dramatically increased in recent years and rapid detection of fungi using nucleic acid-based amplification techniques is helpful in management.