Open AccessFormulation and evaluation of long circulating liposomal Amphotericin B: A scinti-kinetic study using99mTc in BALB/C mice
Author(s) -
M.G. Jadhav,
Mangal S. Nagarsenker,
R. V. Gaikwad,
Abdul Samad,
Nilima A Kshirsagar
Publication year - 2011
Publication title -
indian journal of pharmaceutical sciences/indian journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.245
H-Index - 57
eISSN - 1998-3743
pISSN - 0250-474X
DOI - 10.4103/0250-474x.89757
Subject(s) - liposome , chemistry , amphotericin b , factorial experiment , radiochemistry , chromatography , antifungal , biochemistry , mathematics , biology , microbiology and biotechnology , statistics
In the present study, we formulated long circulating liposomes for amphotericin B and characterized them. The formulation was optimized using 2(3) factorial designs. Pegylated liposomal formulation showed favorable results with reference to particle size (247.33±9.60 nm), percent entrapment efficiency (94.55±3.34%). TEM studies revealed that the liposomes were essentially spherical, hollow, and appeared like powder puff structures. From DSC study it was concluded that the pegylated formulation containing Amp B showed better stability and membrane integrity of the formulation. During the stability studies the formulation was found to be stable. When subjected to gamma scintigraphy kinetic tracer studies the formulation showed longer residence time in the blood in BALB/C mice.