Open AccessUtility and limitations of multiplex ligation-dependent probe amplification technique in the detection of cytogenetic abnormalities in products of conception
Author(s) -
Deepti Saxena,
Meenal Agarwal,
Divya Gupta,
Shuchi Agrawal,
Vinita Das,
Shubha R. Phadke
Publication year - 2016
Publication title -
journal of postgraduate medicine/journal of postgraduate medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.405
H-Index - 52
eISSN - 0972-2823
pISSN - 0022-3859
DOI - 10.4103/0022-3859.192664
Subject(s) - multiplex ligation dependent probe amplification , medicine , multiplex , ligation , computational biology , pathology , bioinformatics , genetics , gene , biology , exon
Background and Introduction: Chromosomal abnormality is found in about half of first-trimester abortions. Karyotype is the gold standard to detect chromosomal abnormalities. Multiplex ligation-dependent probe amplification (MLPA) offers advantage over karyotype in terms of lower failure rate, faster turnaround time, and much higher resolution than conventional karyotyping and found to be 98% concordant with conventional karyotype.