Open AccessA comparison between different existing methods used to separate epidermal cells from skin biopsies for autologous transplantation
Author(s) -
Mohsen KhosraviMaharlooei,
Ali Akbar Mohammadi,
Farsi Ali,
Iman Ahrari,
Armin Attar,
Ahmad Monabati
Publication year - 2011
Publication title -
indian journal of dermatology/indian journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.395
H-Index - 36
eISSN - 1998-3611
pISSN - 0019-5154
DOI - 10.4103/0019-5154.91825
Subject(s) - trypsinization , epidermis (zoology) , hemocytometer , staining , transplantation , medicine , dermis , stain , pathology , giemsa stain , trypsin , microbiology and biotechnology , andrology , chemistry , biology , anatomy , surgery , biochemistry , enzyme
Burn surgeons use autologous skin graft technique for patients, but a challenge remains for large surface wounds. Recently, a method was described which used a small piece of skin to cover a 70 times greater surface by spraying epidermal cells on injured skin. We designed a comparative study to find the best method to make an epidermal cell suspension.