Open AccessComparing the efficacy of hematoxylin and eosin, periodic acid schiff and fluorescent periodic acid schiff-acriflavine techniques for demonstration of basement membrane in oral lichen planus: A histochemical study
Author(s) -
Ashwini Pujar,
Treville Pereira,
Avinash Tamgadge,
Sudhir Bhalerao,
Sandhya Tamgadge
Publication year - 2015
Publication title -
indian journal of dermatology/indian journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.395
H-Index - 36
eISSN - 1998-3611
pISSN - 0019-5154
DOI - 10.4103/0019-5154.159626
Subject(s) - acriflavine , stain , staining , periodic acid–schiff stain , h&e stain , basement membrane , pathology , fluorescence , chemistry , eosin , medicine , biochemistry , optics , physics
Basement membrane (BM) is a thick sheet of extracellular matrix molecules, upon which epithelial cells attach. Various immunohistochemical studies in the past have been carried out but these advanced staining techniques are expensive and not feasible in routine laboratories. Although hematoxylin and eosin (H-E) is very popular among pathologists for looking at biopsies, the method has some limitations. This is where special stains come handy.