Open AccessGetuienis dat termoïnhibering en die opheffing daarvan deur suurstof plus kinetien by Great Lakes - slaaisaad verband hou met mitochondriale funksie
Author(s) -
J.G.C. Small
Publication year - 1996
Publication title -
suid-afrikaanse tydskrif vir natuurwetenskap en tegnologie/die suid-afrikaanse tydskrif vir natuurwetenskap en tegnologie
Language(s) - English
Resource type - Journals
eISSN - 2222-4173
pISSN - 0254-3486
DOI - 10.4102/satnt.v15i1.626
Subject(s) - kinetin , lactuca , chloramphenicol , zoology , biology , horticulture , chemistry , biochemistry , explant culture , in vitro , antibiotics
Thermoinhibition of Lactuca sativa L.cv. Great Lakes 659 seeds occurred at 36 ˚C. Thermoinhibition was alleviated by a combination of 100% O₂ and kinetin (10 mg dm⁻³). Chloramphenicol nullified the effect of O₂ plus kinetin. A number of mitochondrial activities (respiration, coupling, certain matrix enzyme activities and incorporation of [³⁵S]- methionine in mitochondrial proteins) were lower in thermoinhibited than in seeds imbibed at 25 ˚C. Treating seeds with plus kinetin at 36 °C restored these mitochondrial activities to the same or in some cases, to higher levels than in 25 °C control seeds