Open Access’n <i>ln vitro<i/> - vloeikamer as model om die hemostatiese funksie van lewende <i>in situ<i/>-endoteelselle te bepaal
Author(s) -
S. M. Meiring,
Harry F. Kotzé,
G. H. J. Pretorius,
P. N. Badenhorst
Publication year - 1995
Publication title -
suid-afrikaanse tydskrif vir natuurwetenskap en tegnologie/die suid-afrikaanse tydskrif vir natuurwetenskap en tegnologie
Language(s) - English
Resource type - Journals
eISSN - 2222-4173
pISSN - 0254-3486
DOI - 10.4102/satnt.v14i4.620
Subject(s) - aorta , endothelium , perfusion , in vitro , umbilical cord , umbilical vein , anatomy , baboon , chemistry , biology , medicine , endocrinology , biochemistry
Cultured human umbilical cord endothelial cells are mostly used to study the response of endothelium to various stimuli. The effect of the culture process on cell function is however, not known and the function of endothelial cells from different anatomical loci differs. In view of this we developed a flow chamber to study the function of fresh endothelium. The flow chamber was designed to accommodate a five cm piece of baboon aorta in a closed circuit where six hours of perfusion of the aorta with Ringer’s lactate/dextrose buffer at 37 °C was maintained by a peristaltic pump