Open AccessDie ultrastrukturele, morfometriese evaluering van die mikrotubulere stelsel in gestimuleerde B-selle van die pankreas
Author(s) -
Anine Crous,
Alexandra Beer,
Eric J. W. Visser
Publication year - 1992
Publication title -
suid-afrikaanse tydskrif vir natuurwetenskap en tegnologie/die suid-afrikaanse tydskrif vir natuurwetenskap en tegnologie
Language(s) - English
Resource type - Journals
eISSN - 2222-4173
pISSN - 0254-3486
DOI - 10.4102/satnt.v11i4.545
Subject(s) - islet , intracellular , in vitro , pancreas , biology , insulin , endocrinology , chemistry , anatomy , microbiology and biotechnology , biochemistry
The intracellular distribution of microtubules in pancreatic B-cells was studied morphometrically to elucidate the positive correlation between microtubular content and the rate of insulin release found by biochemical investigations. Rat islet tissue was glucose stimulated under in vivo and in vitro (isolated islets) conditions and tissue samples taken to represent both phases of the phasic insulin response. Electron micrographs (x40 000) of individual B-cells were assembled into montages to obtain complete cell profiles at high magnification