Open AccessThe effect of Gallium Aluminium Arsenide laser on fibroblast activity: An in vitro dosimetry study
Author(s) -
Susan. Mars,
Anil A. Chuturgoon,
Dhamarai Pillay,
Maurice Mars
Publication year - 1998
Publication title -
south african journal of physiotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.166
H-Index - 3
eISSN - 2410-8219
pISSN - 0379-6175
DOI - 10.4102/sajp.v54i1.600
Subject(s) - fibroblast , laser , in vitro , in vivo , irradiation , materials science , microbiology and biotechnology , nuclear medicine , chemistry , biomedical engineering , andrology , medicine , optics , biology , biochemistry , physics , nuclear physics
The effect of different doses of low intensity laser therapy (L.I.L.T.) on human fibroblasts was investigated to determine the optimal dose required to stimulate fibroblast proliferation. Human fibroblasts were cultured in vitro and irradiated with different energy densities of 83Onm continuous output infra-red laser using a Gallium Aluminium Arsenide laser. The fibroblasts were irradiated on three consecutive days at energy densities, ranging from 0.2 to 5 J.cm2, delivered at an average radiant power of 30 mW, and at a constant distance of lcm from the fibroblasts. Fibroblast activity was assessed on the fourth day using a calorimetric MTT (tetrazolium) cleavage assay. There was a significant increase in fibroblast proliferation at laser treatment energy densities of 0.4 J.cm2 and 5 J.cm2. Difficulties associated with in vivo and in vitro studies of the effect of laser treatment are discussed.